Raw read trimming must reflect laboratory protocol and its artefacts
![Raw read trimming must reflect laboratory protocol and its artefacts](https://www.bioinformatics.cz/wp-content/uploads/2014/06/1402587667-5f902ee433aa002f745249d76c71880a.png)
Adapters from some lab protocols occupy over 150nt of your precious reads.
![Adapters from some lab protocols occupy over 150nt of your precious reads.](https://www.bioinformatics.cz/wp-content/uploads/2013/03/1388153280-355e25b917243635823ed07625327e3e.png)
Detailed analysis reveals distribution of errors, artefacts, helps troubleshooting and tuneup.
![Detailed analysis reveals distribution of errors, artefacts, helps troubleshooting and tuneup.](https://www.bioinformatics.cz/wp-content/uploads/2014/06/1402592335-47e7fb045c0fba7ae69bbd5de7be659b.png)
Especially transcriptomic protocols leave by design adapters in high-qual sequence.
![Especially transcriptomic protocols leave by design adapters in high-qual sequence.](https://www.bioinformatics.cz/wp-content/uploads/2013/03/read_lengths_A_side_8.png)
Once we do proper primer & adapter & MID removal assembly is possible and without artefacts!
![Once we do proper primer & adapter & MID removal assembly is possible and without artefacts!](https://www.bioinformatics.cz/wp-content/uploads/2013/03/read_lengths_A_side_11.png)